Effect of cadmium on the concentration and expression of TNF-α protein in sea urchin Diadema setosum (Leske, 1778)

Autores

DOI:

https://doi.org/10.24275/uam/izt/dcbs/hidro/2020v29n3/Rumahlatu

Palavras-chave:

heavy metal cadmium, Diadema setosum, TNF-α protein, biomonitoring

Resumo

Background: Cadmium (Cd) is a toxic heavy metal present in seawater, and can damage DNA, producing lipid peroxidation, and protein inactivation. Goals: This research aimed to investigate the effect of Cd on the concentration and expression of TNF-α protein in the Diadema setosum. Methods: This study was conducted for 4 weeks in the laboratory of Marine Conservation LIPI Ambon. Experimental Cd concentrations were 0.0, 1.0, 3.0, 6.0, 9.0, and 12.0 μg/L. The experiment was conducted in 6 tanks (100 x 60 x 70 cm3) each one with 7 individuals of D. setosum as 7 biological replicates. The examination of activation and concentration of TNF-α was performed with immunohistochemical techniques as well as indirect ELISA and western blotting at the Laboratory of Medical Physiology of Brawijaya University. The data related to TNF-α protein concentrations were analyzed by one way ANOVA and a Duncan test (p < 0.05). Results: The results showed that Cd treatment significantly increased the levels of TNF α  protein. There is a linear relationship between Cd concentration and TNF-α protein levels. The level of the TNF- α  protein at the concentration of 12 μg/L Cd was the highest. Conclusions: The results of this research on the concentration and presence of TNF-α protein indicate that this approach could be implemented as a biomonitoring model of the exposure to Cd at the molecular level using D. setosum as bioindicator species in marine waters.

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Biografia do Autor

Dominggus Rumahlatu, Pattimura University

Study Program of Biologi Education, Pattimura University, Rank B Kemenristekdikti

Aloysius Duran Corebima, State University of Malang

Department of Biologi, State University of Malang, Rank A Kemenristekdikti

Mohamad Amin, State University of Malang

Department of Biologi, State University of Malang, Rank A Kemenristekdikti

Fatchur Rohman, State University of Malang

Department of Biologi, State University of Malang, Rank A Kemenristekdikti

Referências

Abbas & Lichtman. 2000. Cellular and Molecular Immunology. Philadelphia: Elvesier Saunders. DOI: 10.1016/B978-1-4160-3123-9.50020-6.

Arai, T., F. Rahman, N. Chino & A. Ismail. 2012. Heavy metal concentrations in a tropical EEL Anguilla bicolor bicolor in Peninsular Malaysia, Malaysia. Malaysian Applied Biology 41 (1): 43-46. DOI: 10.1111/J.1095-8649.2011.03154.X

Ashraf, W. 2005. Accumulation of heavy metals in kidney and heart tissues of Epinephelus microdon fish from the Arabian Gulf. Environ Monit Assess, 101 (1-3): 311-316. DOI: 10.1007/s10661-005-0298-4

Cavalcanti, Y. V. N., M. C. A. Brelaz, J. K. de Andrade Lemoine Neves, J. C. Ferraz & V. R. A Pereira. 2012. Role of TNF-Alpha, IFN-Gamma, and IL-10 in the Development of Pulmonary Tuberculosis. Pulmonary Medicine 2012, Article ID 745483: 1-10. DOI: 10.1155/2012/745483

Chatterjee, S., S. Sarkar & S. Bhattacharya. 2014.Toxic Metals and Autophagy. Chem. Res. Toxicol 27 (11): 1887-1900. DOI: 10.1021/tx500264s

Chiarelli, R. & M. C. Roccheri. 2012. Heavy Metals and Metalloids as Autophagy Inducing Agents: Focus on Cadmium and Arsenic. Cells 1: 597-616. DOI: 10.3390/cells1030597

Dermeche, S., F. Chahrour & Z. Boutiba. 2012. Evaluation of the toxicity of metal pollutants on embryonic development of the sea urchin Paracentrotus lividus (Lamarck, 1816) (Echinodermata Echinoidea). Biodiversity Journal 3 (3): 165-172.

Faix, S., Z. Faixova, K. Boldizarova & P. Javorsky. 2005. The effect of long-term high Heavy metal intake on lipid peroxidation of gastrointestinal tissue in sheep. Vet. Med-Czech 50 (9): 401-405.

Gulbins, E. A., K. Jekle, H. Ferlinz, Grassme & F. Lang. 2000. Physiology of apoptosis. Am J Physiol Renal Physiol 279: 605-615. DOI: 10.1152/AJPRENAL.2000.279.4.F605

Jaishankar, M., T. Tseten, N. Anbalagan, B. B. Mathew & K. N. Beeregowda. 2014. Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol 7 (2): 60-72. DOI: 10.2478/intox-2014-0009

Johri, N., G. Jacquillet & R. Unwin. 2010. Heavy metal poisoning: the effects of cadmium on the kidney. BioMetals 23 (5): 783-792. DOI: 10.1007/s10534-010-9328-y

Jomova, K. & M. Valko. 2011. Advances in metal-induced oxidative stress and human disease. Toxicology 283 (2-3): 65-87. DOI: 10.1016/j.tox.2011.03.001

Jung, M.Y. & Y. M. Lee. 2012. Expression Profiles of Heat Shock Protein Gene Families in the Monogonont Rotifer Brachionus koreanus - Exposed to Copper and Cadmium. Toxicol. Environ. Health. Sci 4 (4): 235-242. DOI 10.1007/s13530-012-0141-6

Kersshaw, E. E. & J. S. Flier. 2004. Adipose tissue as an endocrine organ. The Journal of Clinical Endocrinology & Metabolism 89: 2548-2556. DOI: 10.1210/jc.2004-0395

Lequin, R. M. 2005. Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA). Clinical Chemistry 51 (12): 2415-2418. DOI: 10.1373/clinchem.2005.051532

Liu, W., L. Sun, M. Zhong, Q. Zhou, Z. Gong, P. Li, P. Tai & X. Li. 2012. Cadmium-induced DNA damage and mutations in Arabidopsis plantlet shoots identified by DNA fingerprinting. Chemosphere 89: 1048-1055. DOI: 10.1016/j.chemosphere.2012.05.068

López-Bojórquez, L.N., A. Z. Dehesa, & G. Reyes-Terán. 2004. Molecular mechanisms involved in the pathogenesis of septic shock. Archives of Medical Research 35 (6): 465-479. DOI: 10.1016/j.arcmed.2004.07.006

Machida, K., K. T. H. Cheng, C-K. Lai, K-S. Jeng, V. M. -H. Sung & M. M. C. Lai. 2006. Hepatitis C Virus Triggers Mitochondrial Permeability Transition with Production of Reactive Oxygen Species, Leading to DNA Damage and STAT3 Activation. Journal of Virology 80(14): 7199-7207. DOI: 10.1128/JVI.00321-06

Molnar, N. & P. P. Fong. 2012. Toxic Effects of Copper, Cadmium, and Methoxychlor Shown by Neutral Red Retention Assay in Two Species of Freshwater Molluscs. Environmental Pollution & Toxicology Journal 3: 65-71. DOI: 10.2174/1876397901203010065

Ohta, H., Y.Yamauchi, M. Nakakita, H. Tanaka, S. Asami, Y. Seki & H. Yoshikawa. 2000. Relationship between Renal Dysfunction and Bone Metabolism Disorder in Male Rats after Long-Term Oral Quantitative Cadmium Administration. Industrial Healthol 38: 339-355.

Rumahlatu, D., A. D. Corebima, M. Amin & F. Rohman. 2012. Kadmium dan Efeknya terhadap Ekspresi Protein Metallothionein pada Deadema setosum (Echinoidea; Echinodermata) [cadmium and its effect on the expression of Metallothionein protein in Deadema setosum (Echinoidea; Echinodermata)]. Jurnal Penelitian Perikanan 1 (1): 26-35. [In Indonesian].

Sary, A. A. & M. Mohammadi. 2012. Comparison of Mercury and Cadmium Toxicity in Fish species from Marine water. Res. J. Fish & Hydrobiol 7 (1): 14-18.

Shimoda, R., W.W. Achanzar, W.Qu, T.Nagamine, H. Takagi, M. Mori & M. P. Waalkes. 2003. Metallothionein is a potential negative regulator of apoptosis. Toxicological Sciences 73: 394-300. DOI: 10.1093/toxsci/kfg095

Smiri, M., A. Chaoui & E. E. Ferjani. 2010. Interaction between heacy metals and thiol-linked redox reactions in germination. Pakistan Journal of Biological Sciences 13 (18): 877-883. DOI: 10.3923/pjbs.2010.877.883

Swaroop, J. J., D. Rajarajeswari & J. N. Naidu. 2012. Association of TNF-? with insulin resistance in type 2 diabetes Mellitus. Indian J Med Res 135: 127-130. DOI: 10.4103/0971-5916.93435

Valavanidis, A., T. Vlahogianni, M. Dassenakis & M. Scoullos. 2006. Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicology and Environmental Safety 64 (2): 178-189. DOI: 10.1016/j.ecoenv.2005.03.013

Valko, M., H. Morris & M. T. D. Cronin. 2005. Metals, toxicity and oxidative stress. Current Medicinal Chemistry 2005 (12): 1161-1200.

Wei, Z. 2013. Use of Fourier Transform Infrared Spectroscopy to Study Cadmium-Induced Changes in Strongylocentrotus nudus gonad. Life Science Journal 10 (1): 269-276. DOI: 10.1155/2015/570190

WHO. 2007. Health risks of heavy metals from long-range transboundary air pollution. Publications: WHO Regional Office for Europe Scherfigsvej 8 DK-2100 Copenhagen O, Denmark.

Young, J. & M. Hongbao. 2010. Practical Technique of Western Blotting. The Journal of American Science 4(2): 1-4.

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Publicado

2020-08-26

Como Citar

Rumahlatu, D., Corebima, A. D., Amin, M., & Rohman, F. (2020). Effect of cadmium on the concentration and expression of TNF-α protein in sea urchin Diadema setosum (Leske, 1778). HIDROBIOLÓGICA, 29(3). https://doi.org/10.24275/uam/izt/dcbs/hidro/2020v29n3/Rumahlatu

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