Effect of cadmium on the concentration and expression of TNF-α protein in sea urchin Diadema setosum (Leske, 1778)
DOI:
https://doi.org/10.24275/uam/izt/dcbs/hidro/2020v29n3/RumahlatuPalavras-chave:
heavy metal cadmium, Diadema setosum, TNF-α protein, biomonitoringResumo
Background: Cadmium (Cd) is a toxic heavy metal present in seawater, and can damage DNA, producing lipid peroxidation, and protein inactivation. Goals: This research aimed to investigate the effect of Cd on the concentration and expression of TNF-α protein in the Diadema setosum. Methods: This study was conducted for 4 weeks in the laboratory of Marine Conservation LIPI Ambon. Experimental Cd concentrations were 0.0, 1.0, 3.0, 6.0, 9.0, and 12.0 μg/L. The experiment was conducted in 6 tanks (100 x 60 x 70 cm3) each one with 7 individuals of D. setosum as 7 biological replicates. The examination of activation and concentration of TNF-α was performed with immunohistochemical techniques as well as indirect ELISA and western blotting at the Laboratory of Medical Physiology of Brawijaya University. The data related to TNF-α protein concentrations were analyzed by one way ANOVA and a Duncan test (p < 0.05). Results: The results showed that Cd treatment significantly increased the levels of TNF α protein. There is a linear relationship between Cd concentration and TNF-α protein levels. The level of the TNF- α protein at the concentration of 12 μg/L Cd was the highest. Conclusions: The results of this research on the concentration and presence of TNF-α protein indicate that this approach could be implemented as a biomonitoring model of the exposure to Cd at the molecular level using D. setosum as bioindicator species in marine waters.
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