Potential fish species as genotoxicity biomarkers at lake “La Alberca”, Michoacan, Mexico

Authors

  • Olivia Torres-Bugarín Departamento de Investigación, Programa Internacional de Facultad de Medicina,Universidad Autónoma de Guadalajara. Av. Acueducto esquina Montevideo. Col. Lomas del Valle. 3ra sección. CP 44100 tel (33)3648-8383, ext 3152. E-mail: oliviatorres@hotmail.com
  • José Luis Zavala-Aguirre Escuela de Biología, Facultad de Ciencias, Universidad Autónoma de Guadalajara.
  • Paulina Gómez-Rubio Departamento de Investigación, Programa Internacional de Facultad de Medicina,Universidad Autónoma de Guadalajara. Av. Acueducto esquina Montevideo. Col. Lomas del Valle. 3ra sección. CP 44100 tel (33)3648-8383, ext 3152. E-mail: oliviatorres@hotmail.com
  • Héctor René Buelna-Osben Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Michoacán, Instituto Politécnico Nacional. Becario de COFAA-IPN.
  • Guillermo Zúñiga-González Laboratorio de Mutagénesis, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social.
  • Manuel García-Ulloa Gómez Departamento de Acuacultura, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guadalajara.

Keywords:

Micronuclei, fish, biomonitors, genotoxic, lake of Michoacán.

Abstract

The presence of spontaneous micronuclei in peripheral blood erythrocytes from 10 fish species in Lake La Alberca, Michoacan (Mexico), was evaluated as a possible biological indicator of genotoxic agents. The peripheral blood samples from each of 56 fishes were analyzed by fluorescence microscopy (100X) to determine frequency of micronucleated erythrocytes (EMN) in 10,000 cells, and polychromatic erythrocytes in 1,000 cells (EPC). The cytoplasm-nucleus ratio in erythrocytes (RC/N) also was calculated. The sampled species and their results were: Xenotoca melanosoma (3.7±1.6 EMN, 29.5±15 EPC, 1.7:1 RC/N), Oreochromis aureus (2.0±1.0 EMN, 21.0±14 EPC, 2.6:1 RC/N), Chirostoma consocium (1.5±0.7 EMN, 19.8±14 EPC, 1.4:1 RC/N), Chirostoma lucius (1.2±1.3 EMN, 34.2±19 EPC, 1.8:1 RC/N), Lepomis macrochirus (1.2±1.6 EMN, 10.3±19 EPC, 2.2:1 RC/N), Alloophorus robustus (1.0±1.5 EMN, 31.1±23 EPC, 1.9:1 RC/N), Zoogoneticus quitzeoensis (0.8±1.2 EMN, 23.8±6.3 EPC, 1.6:1 RC/N), Chapalichthys encaustus (0.7±1.0 EMN, 44.6±28 EPC, 1.9:1 RC/N), Poeciliopsis infans (0.7±0.8 EMN, 12.4±4.4 EPC, 1.8:1 RC/N) and Goodea atripinnis (0.6±1.1 EMN, 11.7±5.7 EPC, 1.7:1 RC/N). The frequency of spontaneous EMN found in Xenotoca melanosoma and Oreochromis aureus suggests that these species can be considered as potential biological indicators of genotoxic agents.

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Published

2017-01-15

How to Cite

Torres-Bugarín, O., Zavala-Aguirre, J. L., Gómez-Rubio, P., Buelna-Osben, H. R., Zúñiga-González, G., & García-Ulloa Gómez, M. (2017). Potential fish species as genotoxicity biomarkers at lake “La Alberca”, Michoacan, Mexico. HIDROBIOLÓGICA, 17(1), 75–81. Retrieved from https://hidrobiologica.izt.uam.mx/index.php/revHidro/article/view/967

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