Antibacterial activity in the hemolymph of the catarina scallop Argopecten ventricosus

  • Antonio Luna-González Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Km. 1 Carretera a Las Glorias, Guasave, Sinaloa, México. C. P. 81101. E-mail: aluna@ipn.mx
  • Alfonso Maeda-Martínez Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Mar Bermejo 195. Col. Playa Palo de Santa Rita. La Paz, B.C.S., 23090 México.
  • Ángel Campa-Córdova Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Mar Bermejo 195. Col. Playa Palo de Santa Rita. La Paz, B.C.S., 23090 México.
  • Javier Orduña-Rojas Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Km. 1 Carretera a Las Glorias, Guasave, Sinaloa, México. C. P. 81101. E-mail: aluna@ipn.mx
Palabras clave: Antimicrobial peptide, Argopecten ventricosus, hemolymph, immunity, Vibrio alginolyticus.

Resumen

We conducted a search for antibacterial peptide like activity in hemolymph of Argopecten ventricosus. Pre-purification of peptides was done by reverse phase HPLC. Hemolymph acidic supernatant was loaded into a column packed with C18 matrix. Stepwise elutions were performed with 5, 50, 80, and 100 % acetonitrile (ACN) in 0.05 % trifluoroacetic acid (TFA) over 550 min at a flow rate of 0.2 ml/min. Absorbance was monitored at 280 nm. Eluted fractions were concentrated under vacuum. Vibrio alginolyticus 138-2 was used as a model to test fractions. Growth inhibition zone were observed at 5 and 50 % ACN. This is the first report of antimicrobial peptide-like activity in hemolymph of a pectinid species.

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Publicado
15-01-2017
Cómo citar
Luna-González, A., Maeda-Martínez, A., Campa-Córdova, Ángel, & Orduña-Rojas, J. (2017). Antibacterial activity in the hemolymph of the catarina scallop Argopecten ventricosus. HIDROBIOLÓGICA, 17(1), 87-89. Recuperado a partir de https://hidrobiologica.izt.uam.mx/index.php/revHidro/article/view/969
Sección
Nota Científica