Eficiencia de lectinas como inmunoindicadores en juveniles de camarón blanco, Litopenaeus vannamei (Malacostraca: Penaeidae)

  • Angel Isidro Campa Cordova Centro de Investigaciones Biológicas del Noroeste, S.C.
Palabras clave: anticuerpos monoclonales, inmunoestimulantes, lectinas, Litopenaeus vannamei

Resumen

 Antecedentes: Las lectinas actúan como unidades de reconocimiento para facilitar la eliminación de pató­genos invasivos en plantas y animales, participan en mecanismos de defensa y actúan como mediadores de reconocimiento en la respuesta inmune de invertebrados. Existen pocos estudios dirigidos a evaluar el efecto de inmunoestimulantes sobre la activación de lectinas en crustáceos. Objetivos: Evaluar la concentración de lectina en juveniles de camarón blanco tratados con inmunoestimulantes de origen microbiano y comer­cial. Métodos: Se utilizaron cinco tratamientos agregados directamente al agua de cultivo cada tercer día durante 12 días: 1) laminarina (0.5 mg ml-1); 2) mezcla 1 (Bacillus tequilensis y B. licheniformis; 2 x 106 UFC ml-1, proporción 1:1); 3) mezcla 2 (B. endophyticus, cepa YC3-B y cepa C2-2; 2 x 106 UFC ml-1, proporción 1:1); 4) Debaryomyces hansenii (1 x 106 UFC ml-1); 5) control (sin inmunoestimulantes). Después de los 12 días de tratamiento se tomaron muestras de hemolinfa a las 24, 48 y 72 h para la cuantificación de lectina en plasma, para ello se utilizaron anticuerpos monoclonales contra la lectina de Macrobrachium rosenbergii (MrL). Resultados: A las 24 h posteriores a la última aplicación del tratamiento, se registraron diferencias significativas (p < 0.05) en la concentración de lectina en el plasma de L. vannamei de los camarones ex­puestos al inmunoestimulante comercial laminarina respecto al grupo control, seguida por la mezcla 1 y la mezcla 2 a las 72 horas después de la aplicación de los tratamientos. A las 48 h no hubo diferencias signi­ficativas (p > 0.05) entre los tratamientos y el grupo control. No se encontraron diferencias significativas en los camarones expuestos a la cepa de D. hansenii respecto al grupo control. Conclusiones: Se observó que la concentración de lectina en el plasma de camarón blanco puede incrementarse por el uso de inmunoesti­mulantes y utilizarse como herramienta bioindicadora de inmunoestimulación.

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Biografía del autor/a

Angel Isidro Campa Cordova, Centro de Investigaciones Biológicas del Noroeste, S.C.
Departamento de Acuicultura. Investigador Titular

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Publicado
11-12-2018
Cómo citar
Campa Cordova, A. I. (2018). Eficiencia de lectinas como inmunoindicadores en juveniles de camarón blanco, Litopenaeus vannamei (Malacostraca: Penaeidae). HIDROBIOLÓGICA, 28(3). Recuperado a partir de https://hidrobiologica.izt.uam.mx/index.php/revHidro/article/view/1173
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