Isolation and characterization of new microsatellite markers for the Pacific geoduck (Panopea generosa ) using next generation sequencing

Celia Isabel Bisbal Pardo, Miguel Angel Del Río Portilla, Ana Yonori Castillo Paez, Axayacatl Rocha Olivares

Resumen


Panopea generosa is a large and long-lived infaunal clam with a considerable commercial value in Canada, United States and Mexico. Here, we tested 30 microsatellite loci generated using next-generation genome sequencing (Illumina Hi-Seq 2500) and identified eight as suitable polymorphic genetic markers. The number of alleles per locus ranged from 5 to 22 and heterozygosity from 0.429 to 0.818 (observed) and from 0.548 to 0.962 (expected). These markers are highly informative and useful for population genetic studies aimed at informing management and conservation measures of this valuable resource.


Palabras clave


Microsatellite, Panopea generosa, Next-generation sequencing, genetic markers

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DOI: https://doi.org/10.24275/uam/izt/dcbs/hidro/2018v28n1/Rocha

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