Structure of the mitochondrial control region and flanking tRNA genes of Mugil cephalus

Autores/as

  • Axayácatl Rocha-Olivares Department of Biological Oceanography, CICESE, km 107 Carretera Tijuana-Ensenada, Ensenada B. C. 22860
  • Nikola M. Garber NOAA Sea Grant, 1315 East West Highway, R/SG, Rm 11718, Silver Spring, Maryland 20910
  • Amber F. Garber North Carolina State University, Department of Zoology, Campus Box 7617, Raleigh, North Carolina 27695-7617
  • Kenneth C. Stuck Gulf Coast Research Laboratory, University of Southern Mississippi, PO Box 7000, Ocean Springs, Mississippi 39566-7000 Dr. Axayácatl Rocha-Olivares CICESE-Department of Biological Oceanography Tel: (646) 175-0500 x 24240 Fax: (646) 175-0545

Palabras clave:

Mitochondrial DNA, cloning, molecular divergence, mullet, control region.

Resumen

We cloned and sequenced the mitochondrial (mt) control region (CR) and flanking transfer RNA genes (T, P, and F) of the striped mullet, Mugil cephalus and designed species-specific primers to amplify the entire CR in specimens from the Pacific (Hawaii), the Gulf of Mexico, and the Atlantic. We verified the absence of heteroplasmy and nuclear mtDNA duplications of this region in the organisms sampled, finding an extraordinary level of sequence divergence (mean 38-75% Tamura & Nei distance- ) between fish from both Oceans, including Japan. The CR of the mullet was variable in length (845-930 bp) and contained structural elements in common with other CRs, including a central conserved segment flanked by hypervariable regions and smaller conserved sequence blocks. Termination associated sequences, however, were not found. The CR of the striped mullet was rich in AT (~67%) and poor in GC.

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Publicado

2017-01-17

Cómo citar

Rocha-Olivares, A., Garber, N. M., Garber, A. F., & Stuck, K. C. (2017). Structure of the mitochondrial control region and flanking tRNA genes of Mugil cephalus. HIDROBIOLÓGICA, 15(2), 139–149. Recuperado a partir de https://hidrobiologica.izt.uam.mx/index.php/revHidro/article/view/1059