Segregación de las variedades roja y Stirling de Oreochromis niloticus y del híbrido O. aureus x O. niloticus (Pisces:Cichlidae) mediante la lectina algal "Giraffina"

Authors

  • Sergio Alvarez-Hernández Laboratorio de Ficología Aplicada.
  • Graciela De Lara-Isassi Laboratorio de Ficología Aplicada.
  • José Luis Arredondo-Figueroa Planta Experimental de Producción Acuícola. Departamento de Hidrobiología. Universidad Autónoma Metropolitana-Iztapalapa. Apartado Postal 55-535. México, D.F., C.P. 09340.
  • Ricardo Campos Verduzco Planta Experimental de Producción Acuícola. Departamento de Hidrobiología. Universidad Autónoma Metropolitana-Iztapalapa. Apartado Postal 55-535. México, D.F., C.P. 09340.

Keywords:

lectin, Giraffine, segregation, Codium giraffa, Oreochromis

Abstract

In order to separate two strains of tilapia (genus oreochromis) of O. niloticus (red and stirling strains) and rocky mountain hybrid (O. aureus X O. niloticus) the lectin giraffine isolated from Codium giraffa (Chlorophyta) was used. Fish blood was obtained by cardiac puncture and prepared as a 2 % suspension in phosphate buffer 100 mN, pH 7.2. At all cases, the lctin giraffine agglutinated the fish bood. The statical analysis of agglutination results showed significantly differences (p?0.05) at single classification analysis of variance. Multiple comparisions among means SNK test shows overlapping between red and stirling strains, and could separate the hybrid rocky mountain from this group. We can concluded that giraffine can be used as an easy and cheap test for grouping these fishes. This test can aid the researcher to follow correctly the progeny of this fishes, commercially valuable as a food source in Mexico.

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Published

2017-01-16

How to Cite

Alvarez-Hernández, S., De Lara-Isassi, G., Arredondo-Figueroa, J. L., & Campos Verduzco, R. (2017). Segregación de las variedades roja y Stirling de Oreochromis niloticus y del híbrido O. aureus x O. niloticus (Pisces:Cichlidae) mediante la lectina algal "Giraffina". HIDROBIOLÓGICA, 11(2), 117–121. Retrieved from https://hidrobiologica.izt.uam.mx/hidrobiologica/index.php/revHidro/article/view/960

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