Vibrio parahaemolyticus strains causing acute hepatopancreatic necrosis disease in farming shrimp of Sonora, Mexico and their antibiotic resistance
DOI:
https://doi.org/10.24275/uam/izt/dcbs/hidro/2021v31n2/GalavizKeywords:
bearly mortality syndrome, Litopenaeus vannamei, shrimp, Vibrio parahaemolyticus AHPNDAbstract
Background: Shrimp farms in northwestern Mexico have had outbreaks since 2013 with losses of more than 80%. The only background is the acute hepatopancreatic necrosis diseases (AHPND) originated in Asia for a new strains of Vibrio parahaemolyticus (VP-AHPND+) that produce toxins PirA and PirB of the plasmid pVA1. Goals: To characterize the causative agent of epizootics in farms; to perform genomic comparisons of sequencing of the 16S rDNA region to identify the variability that occurs in the strains and evaluate the resistance developed against antibiotics in vitro. Methods: Estuaries, drains, and farms of southern Sonora were monitored; samples of water, sediment and shrimps were aseptically sampled. Bacterial cultures were performed in TCBS and the green colonies were streaked in the selective culture media CHROMagar Vibrio medium. Bacteria were identified by PCR-AP3 specific for the strains VP-AHPND+. Polymorphism of strains were analyzed by sequencing the 16S rDNA region and the rep-PCR (GTG)5 technique. Antibiotic resistance was examined for oxitetracicline, florfenicol, enrofloxacin, magnacin, sulfacloropiridacin trimetroprim and enrofloxacin. Results: Throughout 2013-2014, 444 strains were isolated (82 from 2013, and 362 from 2014). Eighty-three were selected, from which 16 were PCR-positives. Sequences from 16S rDNA region were compared with those deposited at GenBank, and a high homology was found with sequences from VP-AHPND+ from Sinaloa with differences of those from Thailand and China, polymorphism occur mainly in 15 nucleotide sequences. Regards to antibiotic resistance tests, strains with resistance to oxytetracycline and sulfachloropyridazine-trimethoprim were found. Conclusions: VP-AHPND+ was identified as the causative agent of AHPND in shrimp farms from Sonora and the polymorphism among the strains was confirmed, also antibiotic resistance was found against antibiotics, regularly applied to shrimp farms.
Downloads
References
Alday-Sanz, V., A. Roque & J. F. Turnbull. 2002. Clearing mechanisms ofVibrio vulnificus biotype I in the black tiger shrimp Penaeus monodon. Diseases of Aquatic Organisms 48: 91-99. DOI: 10.3354/dao048091.
Aranguren-Caro, L. F., H. N. Mai, B. Noble & A. K. Dhar. 2020. Acute hepatopancreatic necrosis disease (VPAHPND), a chronic disease in shrimp (Penaeus vannamei) population raised in latin America. Journal of Invertebrate Pathology 174: 107424.
CESASIN (Comité Estata l de Sanidad Acuícola de Sinaloa). 2020. Informacion Sanitaria 2020. Disponible en línea en: https://cesasin.mx/programacrustaceos/# estatsanit (consultado el 06 enero 2021).
CLSI (Clinical and Laborat ory Sta ndards Institute). 2005a. Methods for Antimicrobial Disk Susceptibility Testing of bacteria isolated from aquatic animals; proposed guideline. CLSI document M42-P. Clinical and Laboratory Standards Institute, USA. 39 p.
CLSI (Clinical and Laborat ory Sta ndards Institute). 2005b. Methods for broth dilution susceptibility testing of bacteria isolated from aquatic animals; proposed guideline. CLSI document M49-P. Clinical and Laboratory Standards Institute, USA. 43 p.
CONAPESCA (Comisión Nacional de Pesca y Acuacultura). 2018. Cultivo de camaron, produccion acuicola de calidad. Disponible en línea en: https://www.gob.mx/conapesca/articulos/cultivo-de-camaron- produccion-acuicola-de-calidad?idiom=es (consultado el 19 octubre 2018).
COSAES (Comité de Sanidad Acuícola del Esta do de Sonora, A.C.). 2017. Informe final sanidad camaron. Disponible en linea en: https://docs.wixstatic.com/ugd/e56b21_b08ea49c19074c97993c9c-9622d263f5.pdf (consultado el 15 mayo 2018).
Chávez-Sánchez, C. & I. Higuera-Ciapara . 2003. Manual de buenas practicas de produccion acuicola de camaron para la inocuidad alimentaria. SAGARPA, SENASICA. Mazatlan, Mexico. 97 p.
Dhar, A. K., P. Piamsomboon, L. F. Aranguren-Caro, S. Kanrar, R. Adami-Jr & Y. S. Juan. 2019. First report of acute hepatopancreatic necrosis disease (AHPND) occurring in the USA. Diseases of aquatic organisms 132 (3): 241-247.
Di-Pinto, A., V. Terio, L. Novello & G. Tantillo. 2011. Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus. Food Control 22: 124-127.
Dong, X., H. Wang, P. Zou, J. Chen, Z. Liu, X. Wang & J. Huang. 2017. Complete genome sequence of Vibrio campbellii strain 20130629003S01 isolated from shrimp with acute hepatopancreatic necrosis disease. Gut Pathogens 9 (31): 1-5. DOI: 10.1186/s13099-017-0180-2.
FAO (Food and Agriculture Organizat ion). 2014. El estado mundial de la pesca y la acuicultura. Organizacion de las Naciones Unidas para la Alimentacion y la Agricultura, Roma. 274 p.
Flegel, T. W. 2012. Historic emergence, impact and current status of shrimp pathogens in Asia. Journal of Invertebrate Pathology 110: 166-173.
Flegel, T. W., D. V. Lightner, C. F. Lo & L. Owens. 2008. Shrimp disease control: past, present and future. In: Bondad-Reantaso, M. G., C. V. Mohan, M. Crumlish & R. P. Subasinghe (eds.). Diseases in Asian Aquaculture VI. Fish Health Section, Asian Fisheries Society, Manila, Philippines, pp. 355-378.
Galav iz-Silva , L., J. Iracheta -Villarreal & Z. J. Molina-Garza. 2018. Bacillus and Virgibacillus strains isolated from three Mexican coasts antagonize Staphylococcus aureus and Vibrio parahaemolyticus. FEMS Microbiology Letters 365: fny202. DOI:10.1093/femsle/fny202
García, F. 2020. Produccion de camaron crece para alimentar a Mexico. Revista Divulgación Acuícola. Disponible en linea en http://divulgacionacuicola.com.mx/blog/2020/12/15/produccion-de-camaron-
crece-para-alimentar-a-mexico/ (consultado el 8 de enero 2021).
Gomez-Gil, B., L. Tron-Mayén, A. Roque, J. F. Turnbull, V. Inglis & A. L. Guerra-Flores. 1998. Species of Vibrio isolated from hepatopancreas, haemolymph and digestive tract of a population of healthy juvenile Penaeus vannamei. Aquaculture 163:1-9.
González‑Gómez, J. P., S. Soto‑Rodriguez, O. López‑Cuevas , N. Castro del Campo, C. Chaidez & B. Gomez-Gil. 2020. Phylogenomic analysis supports two possible origins for Latin American Strains of Vibrio parahaemolyticus associated with Acute Hepatopancreatic Necrosis Disease (AHPND). Current Microbiology 77 (12): 3851-3860. DOI:10.1007/s00284-020-02214-w.
Han, J. E., L. L. Mohney, K. F. J. Tang, C. R. Pantoja & D. V. Lightner. 2015. Plasmid mediated tetracycline resistence of Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND) in shrimps. Aquacultur Reports 2:17-21. DOI:10.1016/j.aqrep.2015.04.003
Hastuti, M. S. & Desrina. 2016. Current status of acute hepatopancreatic necrosis disease (AHPND) and other transboundary diseases of farmed shrimps in Indonesia. In: Pakingking-Jr, R. V. E., G. T. de Jesus-Ayson & B. O. Acosta (eds.). Addressing Acute Hepatopancreatic Necrosis Disease (AHPND) and Other Transboundary Diseases for Improved Aquatic Animal Health in Southeast Asia: Proceedings of the ASEAN Regional Technical Consultation on EMS/AHPND and Other Transboundary Diseases for Improved Aquatic Animal Health in Southeast Asia, 22-24 February 2016. Southeast Asian Fisheries Development Center Aquaculture Department Tigbauan, Iloilo, Makati City, Philippines, pp. 37-43.
Joshi, J., J. Srisala, V. H. Truong, I. T. Chen, B. Nuangsaeng, O. Suthienkul, C. F. Lo, T. W. Flegel, K. Sritunyalucksana & S. Thitama dee. 2014. Variation in Vibrio parahaemolyticus isolates from a single Thai shrimp farm experiencing an outbreak of acute hepatopancreatic necrosis disease (AHPND). Aquaculture 428: 297-302.
Kondo, H., S. Tinwongger, P. Proespraiwong, R. Mav ichak, S. Unajak, R. Nozaki & I. Hirono. 2014. Draft genome sequences of six strains of Vibrio parahaemolyticus isolated from early mortality syndrome/acute hepatopancreatic necrosis disease shrimp in Thailand. Genome Announcements 2: e00221-14 Lightner, D. V. 2011. Virus diseases of farmed shrimp in the Western Hemisphere (The Americas). Journal of Invertebrate Pathology 106: 110-130.
Lightner, D. V. & R. M. Redman. 1998. Shrimp diseases and current diagnostic methods. Aquaculture 164: 201-220.
Lightner, D.V., R. M. Redman, C. R. Pantoja, B. L. Noble & L. Tran. 2012. Early mortality syndrome affects shrimp In Asia. Global Aquaculture Advocate. January/February 15(1): 40.
Liu, L., J. Xiao, X. Xia, Y. Pan, S. Yan & Y. Wang. 2015. Draft genome sequence of Vibrio owensii strain SH-14, which causes shrimp acute hepatopancreatic necrosis disease. Genome Announcements
(6):e01395-15.
NOM-EM-05-PESC-2002 (Norma Oficial Mexicana). 2002. Que establece los requisitos y medidas para prevenir y controlar la dispersión de enfermedades de alto impacto y para el uso y aplicación de antibióticos en la camaronicultura nacional. Diario Oficial de la Federación. Secretaria de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación México, D.F. Julio 19.
NOM-230-SSA1-2002 (Norma Oficial Mexicana). 2002. Salud ambiental. Agua para uso y consumo humano. Requisitos sanitarios que se deben cumplir en los sistemas de abastecimiento públicos y privados durante el manejo del agua. Procedimientos sanitarios para el muestreo. Disponible en línea en: http://www.salud.gob.mx/unidades/cdi/nom/230ssa102.html (consultado el 18 agosto 2018).
Matsh eka, M. I., A. J. Lastovica, H. Zappe & B. G. Elisha. 2005. The use of (GTG)5 oligonucleotide as an RAPD primer to type Campylobacter concisus. Letters in Applied Microbiology 42: 600-605.
Muthukrishnan, S., T. Defoirdt, M. Y. Ina-Salwa nya, F. M. Yusoff, M. Shariff, S. I. Ismail & I. Natrah . 2019. Vibrio parahaemolyticus and Vibrio harveyi causing Acute Hepatopancreatic Necrosis Disease (AHPND) in Penaeus vannamei (Boone, 1931) isolated from Malaysian shrimp ponds. Aquaculture 511: 734227. DOI:10.1016/j.aquaculture. 2019.734227
OIE (Oficina Internacional de Epizootias). 2013. Capitulo 1.4. Vigilancia de la Sanidad de los Organismos Acuaticos. Codigo Sanitario para los Animales Acuaticos. Disponible en linea en: http://www.oie.int/es/normas-internacionales/codigo-acuatico/acceso-en-linea/ (consultado el 24 julio 2018).
Restrepo, L., B. Bayot, S. Arciniegas, L. Bajaña, I. Beta ncourt , F. Panchana & A. Reyes-Muñoz. 2018. PirVP genes causing AHPND identified in a new Vibrio species (Vibrio punensis) within the commensal Orientalis clade. Scientific reports 8: 13080. DOI:10.1038/s41598-018-30903-x
Rivas , R., E. Velazquez, J. L. Zurdo-Piñeiro, P. F. Mat eos & E. Molina. 2004. Identification of microorganisms by PCR amplification and sequencing of a universal amplified ribosomal region present in both prokaryotes and eukaryotes. Journal of Microbiological Methods 56: 413-426. DOI:10.1016/j.mimet.2003.11.007
Rorta na, C., W. Wajjwa lku, V. Boonyaw iwat , C. Hrianpreecha, S. Thongratsak ul & P. Amav isit. 2018. Antimicrobial resistance and pirAB-like profiles of Vibrio parahaemolyticus in Pacific white shrimp. Agriculture and
Natural Resources 54: 377-381. DOI:10.1016/j.anres.2018.10.010
Santiago, H., A. Espinosa & M. C. Bermúdez. 2009. Uso de antibioticos en la camaronicultura. Revista Mexicana de Ciencias Farmacéuticas 40: 22-32.
Saitou, N. & M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees MEGA 7. Molecular Biology and Evolution 4: 406-425.
SENASICA (Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimenta -ria). 2014. Conferencia: “Sindrome de la mortalidad temprana (EMS) del camaron”. Disponible en linea en: https://www.gob.mx/ senasica/prensa/conferencia-sindrome-de-la-mortalidad-temprana-ems-del-camaron) (consultado el 06 enero 2021).
Sirikharin, R., S. Taengchaiyaphum, P. Sanguanrut, T. D. Chi, R. Mav ichak, P. Proespraiwong, B. Nuangsaeng, S. Thitama dee, T. W. Flegel & K. Sritunyalucksana. 2015. Characterization and PCR detection of binary, pir-like toxins from Vibrio parahaemolyticus isolates that cause acute hepatopancreatic necrosis disease (AHPND) in shrimp. PLoS ONE 10: e0126987. DOI:10.1371/journal.pone.0126987
Soto-Rodriguez, S. A., B. Gomez-Gil & R. Lozano. 2010. Density of Vibrios in hemolymph and hepatopancreas of diseased pacific white shrimp, Litopenaeus vannamei, from Northwestern Mexico. Journal of the World Aquaculture Society 41: 76-83
Soto-Rodriguez, S. A., B. Gomez-Gil, R. Lozano-Olvera, M. Betancourt –Lozano & M. S. Morales-Covarrubias. 2015. Field and experimental evidence of Vibrio parahaemolyticus as the causative agent of acute hepatopancreaticnecrosis disease of cultured shrimp (Litopenaeus vannamei) in northwestern Mexico. Applied Environments Microbiology 81:1689-1699. DOI:10.1128/AEM.03610-14
Tran, L., L. Nunan, R. M. Redman, L. L. Mohney, C. R. Pantoja, K. Fitzsimmons & D. V. Lightner. 2013. Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Diseases of Aquatic Organisms 105: 45-55.
Varela-Mejía, A. & R. Alfar o-Mora. 2018. Revisión sobre aspectos farmacológicos a considerar para el uso de antibióticos en la camaronicultura. Revista de Investigación en Perú 29: 1-14. DOI:10.15381/
rivep.v29i1.14186
Yang, Q., X. Dong, G. Xie, S. Fu, P. Zou, J. Sun & J. Huang. 2019. Comparative genomic analysis unravels the transmission pattern and intra-species divergence of acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strains. Molecular Genetics and Genomics 294: 1007-1022.
DOI: 10.1007/s00438-019-01559-7
Yano, Y., K. Hamano, M. Sat omi, I. Tsutsui, M. Ban, D. Aueumneoy. 2014. Prevalence and antimicrobial susceptibility of Vibrio species related to food safety isolated from shrimp cultured at inland ponds in Thailand. Food Control 38: 30-36.
Downloads
Published
How to Cite
Issue
Section
License
Los autores/as que publiquen en esta revista aceptan las siguientes condiciones:
De acuerdo con la legislación de derechos de autor, HIDROBIOLÓGICA reconoce y respeta el derecho moral de los autores, así como la titularidad del derecho patrimonial, el cual será cedido a la revista para su difusión en acceso abierto.
Publicar en la revista HIDROBIOLÓGICA tiene un costo de recuperación de $500 pesos mexicanos por página en blanco y negro (aproximadamente 29 dólares americanos) y $1000 pesos por página a color (aproximadamente 58 dólares americanos).
Todos los textos publicados por HIDROBIOLÓGICA sin excepción se distribuyen amparados bajo la licencia Creative Commons 4.0Atribución-No Comercial (CC BY-NC 4.0 Internacional), que permite a terceros utilizar lo publicado siempre que mencionen la autoría del trabajo y a la primera publicación en esta revista.
Los autores/as pueden realizar otros acuerdos contractuales independientes y adicionales para la distribución no exclusiva de la versión del artículo publicado en HIDROBIOLÓGICA (por ejemplo incluirlo en un repositorio institucional o publicarlo en un libro) siempre que indiquen claramente que el trabajo se publicó por primera vez en HIDROBIOLÓGICA.
Para todo lo anterior, el o los autor(es) deben remitir el formato de Carta-Cesión de la Propiedad de los Derechos de la primera publicación debidamente requisitado y firmado por el autor(es). Este formato se puede enviar por correo electrónico en archivo pdf al correo: enlacerebvistahidrobiológica@gmail.com; rehb@xanum.uam.mx (Carta-Cesión de Propiedad de Derechos de Autor).
Esta obra está bajo una licencia de Creative Commons Reconocimiento-No Comercial 4.0 Internacional.
