A simple method for short-term storage and transportation of spermatophores of Pacific white shrimp (Litopenaeus vannamei )

Karina Morales-Ueno, Carmen G. Paniagua-Chávez, Alfonso Martínez-Ortega, Héctor Castillo-Juárez, Jorge Alfaro-Montoya

Resumen


The development of a shipping method for spermatophores of the white shrimp Litopenaeus vannamei would open new opportunities for sharing and improving genetic resources of shrimp worldwide. Seventy spermatophores were collected daily for 5 days (a total of 350 spermatophores from 175 shrimp), packed in microcentrifuge tubes containing 100 μL of an extender solution, and placed in a Styrofoam box supplied with a thermal insulating layer and refrigerant pack to keep the samples cooled at ~14°C. Shipment of samples took ~26 hours. At arrival, spermatophores were randomly sampled either as soon as the box arrived (~27 h, Group A) or five hours later (~32 h, Group B) to assess sperm viability. Spermatozoal morphology was evaluated by microscopy (100 cells per shrimp). Cells without spikes or irregular in shape were recorded as abnormal; otherwise cell morphology was recorded as normal. Spermatozoal viability was assessed by flow cytometry, whereby three populations were identified: (1) cells with intact cytoplasmatic membrane (viable), (2) cells with disrupted membrane (non-viable), and (3) cells in transition, changing from intact to disrupted membrane (transitional). Significant differences were found in spermatozoal morphology between group A and B (p = 0.002), with the highest percentage of normal spermatozoa (92 + 15%) found in Group A. No significant differences were found in viable (p = 0.723) and transitional spermatophore populations (p = 0.595) assessed by flow cytometry. Non-viable populations increased with time in storage (p = 0.039). The highest percentage of non-viable cells (81 + 7%) was obtained in Group B. These results indicate that spermatophores can be cooled and transported to distant locations maintaining normal morphology and viability. These indirect quality indicators suggest that spermatozoa may be used for different purposes, including artificial insemination.

Palabras clave


Camarón; espermatóforos; Litopenaeus vannamei; transporte.

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ISSN 0188-8897

 HIDROBIOLOGICA. Vol.26 Año 2016, Número 3, septiembre-diciembre de 2016, es una publicación cuatrimestral editada por la Universidad Autónoma Metropolitana, a través de la Unidad    Iztapalapa, División de Ciencias Biológicas y de la Salud, Departamento de Hidrobiología. Prolongación Canal de Miramontes 3855, colonia Ex Hacienda San Juan de Dios, delegación Tlalpan,  C.P. 14387, México, Ciudad de México y Av. San Rafael Atlixco, núm. 186, colonia Vicentina, delegación Iztapalapa, C.P. 09340, México, Ciudad de México, teléfono 5804-6475. Página  electrónica de la revista: http://hidrobiologica.izt.uam.mx y dirección electrónica: rehb@xanum.uam.mx. Editora Responsable María Esther Meave del Castillo. Certificado de Reserva de  Derechos al Uso Exclusivo de Título No. 04-2010-072711181500-203, ISSN para version impresa e ISSN para revista electronica son otorgados por el Instituto Nacional del Derecho de Autor. Responsable de la última actualización  de este número María Esther Meave del Castillo, Universidad Autónoma Metropolitana Unidad Iztapalapa, Av. San Rafael Atlixco, núm. 186, colonia Vicentina, delegación Iztapalapa, C.P.  09340, México, Ciudad de México. Fecha de última modificación: 2 de marzo de 2017. Tamaño de archivo: 30 MB.Las opiniones expresadas por los autores no necesariamente reflejan la  postura del editor de la publicación.Queda estrictamente prohibida la reproducción total o parcial de los contenidos e imágenes de la publicación sin previa autorización de la Universidad  Autónoma Metropolitana. 

ISSN para versión impresa 0188-8897 

ISSN para version electronica 2448-7333